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kapa library quantification kit  (Roche)


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    Structured Review

    Roche kapa library quantification kit
    Kapa Library Quantification Kit, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/library+quantification+kit/KAPA+Quantification+Kit/pmc12854873-165-8-12
    Average 99 stars, based on 5 article reviews
    kapa library quantification kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Synergistic effects of ferulic acid and wogonin on cholestatic liver injury via gut microbiota modulation.
    Article Snippet: Amplicons were purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, USA), quantified with Qubit (Invitrogen, USA), and pooled in equimolar concentrations. .. Sequencing libraries were constructed, and quality control was performed using the Agilent 2100 Bioanalyzer and the Library Quantification Kit (Kapa Biosystems). ..

    Article Title: Synergistic effects of ferulic acid and wogonin on cholestatic liver injury via gut microbiota modulation
    Article Snippet: Amplicons were purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, USA), quantified with Qubit (Invitrogen, USA), and pooled in equimolar concentrations. .. Sequencing libraries were constructed, and quality control was performed using the Agilent 2100 Bioanalyzer and the Library Quantification Kit (Kapa Biosystems). .. Sequencing was conducted on an Illumina MiSeq platform to generate high-quality paired-end reads (2 × 250 bp).

    Construct:

    Article Title: Synergistic effects of ferulic acid and wogonin on cholestatic liver injury via gut microbiota modulation.
    Article Snippet: Amplicons were purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, USA), quantified with Qubit (Invitrogen, USA), and pooled in equimolar concentrations. .. Sequencing libraries were constructed, and quality control was performed using the Agilent 2100 Bioanalyzer and the Library Quantification Kit (Kapa Biosystems). ..

    Article Title: Synergistic effects of ferulic acid and wogonin on cholestatic liver injury via gut microbiota modulation
    Article Snippet: Amplicons were purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, USA), quantified with Qubit (Invitrogen, USA), and pooled in equimolar concentrations. .. Sequencing libraries were constructed, and quality control was performed using the Agilent 2100 Bioanalyzer and the Library Quantification Kit (Kapa Biosystems). .. Sequencing was conducted on an Illumina MiSeq platform to generate high-quality paired-end reads (2 × 250 bp).

    Control:

    Article Title: Synergistic effects of ferulic acid and wogonin on cholestatic liver injury via gut microbiota modulation.
    Article Snippet: Amplicons were purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, USA), quantified with Qubit (Invitrogen, USA), and pooled in equimolar concentrations. .. Sequencing libraries were constructed, and quality control was performed using the Agilent 2100 Bioanalyzer and the Library Quantification Kit (Kapa Biosystems). ..

    Article Title: Synergistic effects of ferulic acid and wogonin on cholestatic liver injury via gut microbiota modulation
    Article Snippet: Amplicons were purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, USA), quantified with Qubit (Invitrogen, USA), and pooled in equimolar concentrations. .. Sequencing libraries were constructed, and quality control was performed using the Agilent 2100 Bioanalyzer and the Library Quantification Kit (Kapa Biosystems). .. Sequencing was conducted on an Illumina MiSeq platform to generate high-quality paired-end reads (2 × 250 bp).

    Library Quantification:

    Article Title: Synergistic effects of ferulic acid and wogonin on cholestatic liver injury via gut microbiota modulation.
    Article Snippet: Amplicons were purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, USA), quantified with Qubit (Invitrogen, USA), and pooled in equimolar concentrations. .. Sequencing libraries were constructed, and quality control was performed using the Agilent 2100 Bioanalyzer and the Library Quantification Kit (Kapa Biosystems). ..

    Article Title: Clinical, in vitro, and in vivo evidence of WAPL as a novel cohesinopathy gene and phenotypic driver of 10q22.3q23.2 genomic disorder
    Article Snippet: .. QC was undertaken on a TapeStation 4200 (Agilent) and qPCR with a Library Quantification Kit (Kapa Biosystems). .. Finally, libraries were pooled for sequencing on an Illumina NovaSeq6000 using an S4 300 cycles kit.

    Article Title: Synergistic effects of ferulic acid and wogonin on cholestatic liver injury via gut microbiota modulation
    Article Snippet: Amplicons were purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, USA), quantified with Qubit (Invitrogen, USA), and pooled in equimolar concentrations. .. Sequencing libraries were constructed, and quality control was performed using the Agilent 2100 Bioanalyzer and the Library Quantification Kit (Kapa Biosystems). .. Sequencing was conducted on an Illumina MiSeq platform to generate high-quality paired-end reads (2 × 250 bp).

    Article Title: A disease model resource reveals core principles of tissue-specific cancer evolution.
    Article Snippet: After each PCR step, solid-phase reversible immobilization clean-up (0.8×) was performed using an Agencourt AMPure XP kit (Beckman Coulter). .. The pooled library was quantified by SYBR Green qPCR (Sigma-Aldrich) and a Kapa Biosystems library quantification kit (Roche). .. The resulting library was sequenced on a NextSeq 550 (Illumina) system.

    Article Title: Mapping Escherichia coli in Women with Simple Urinary Tract Infections: Phenotypic ESBL/AmpC Screening and Whole-Genome Insights from Oman
    Article Snippet: The following steps were performed at the sequencing facility: DNA libraries were prepared with the Nextera XT Library Prep Kit (Illumina, San Diego, CA, USA) following the manufacturer’s instructions, with two modifications: the input DNA was doubled, and the PCR elongation step was extended to 45 s. DNA quantification and library preparation were performed using a Hamilton Microlab STAR automated liquid-handling platform (Hamilton Bonaduz AG, Bonaduz, Switzerland). .. Pooled libraries were quantified using the Kapa Biosystems Library Quantification Kit (Kapa Biosystems, Wilmington, MA, USA) for Illumina and sequenced on Illumina HiSeq/NovaSeq instruments with a 250 bp paired-end protocol. ..

    Article Title: Mapping Escherichia coli in Women with Simple Urinary Tract Infections: Phenotypic ESBL/AmpC Screening and Whole-Genome Insights from Oman
    Article Snippet: The following steps were performed at the sequencing facility: DNA libraries were prepared with the Nextera XT Library Prep Kit (Illumina, San Diego, CA, USA) following the manufacturer’s instructions, with two modifications: the input DNA was doubled, and the PCR elongation step was extended to 45 s. DNA quantification and library preparation were performed using a Hamilton Microlab STAR automated liquid-handling platform (Hamilton Bonaduz AG, Bonaduz, Switzerland). .. Pooled libraries were quantified using the Kapa Biosystems Library Quantification Kit (Kapa Biosystems, Wilmington, MA, USA) for Illumina and sequenced on Illumina HiSeq/NovaSeq instruments with a 250 bp paired-end protocol. ..

    Article Title: Ionic Regulation of Mechanosurveillance and Metastasis via the MRTFA/KCNMB1 Axis
    Article Snippet: The concentration of the final library pool was confirmed by qPCR and subjected to a test sequencing run on a MiniSeq instrument (Illumina) in order to check sequencing efficiencies and adjust accordingly proportions of individual libraries. .. The adjusted library pool was re-quantified by qPCR using Library Quantification Kit (Kapa Biosystems) and sequenced on the NovaSeq 6000 instrument (Illumina), flowcell, 2×150 bp reads. ..

    Article Title: Adenine base editors with reduced off-target effects
    Article Snippet: Stranded RNA-seq libraries were generated from these samples using the PrepX mRNA 48 kit (Takara) on the Apollo 324 followed by barcoding and amplification (12 cycles). .. Following PCR and bead cleanup with AmpureXP beads (Beckman Coulter), libraries were visualized on a 2200 TapeStation (Agilent) and quantified using a Library Quantification Kit (KAPA Biosystems) for multiplexing. libraries were sequenced on a NextSeq high-throughput flowcell (Illumina) as 150 bp paired-end reads. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Clinical, in vitro, and in vivo evidence of WAPL as a novel cohesinopathy gene and phenotypic driver of 10q22.3q23.2 genomic disorder
    Article Snippet: .. QC was undertaken on a TapeStation 4200 (Agilent) and qPCR with a Library Quantification Kit (Kapa Biosystems). .. Finally, libraries were pooled for sequencing on an Illumina NovaSeq6000 using an S4 300 cycles kit.

    Article Title: A disease model resource reveals core principles of tissue-specific cancer evolution.
    Article Snippet: After each PCR step, solid-phase reversible immobilization clean-up (0.8×) was performed using an Agencourt AMPure XP kit (Beckman Coulter). .. The pooled library was quantified by SYBR Green qPCR (Sigma-Aldrich) and a Kapa Biosystems library quantification kit (Roche). .. The resulting library was sequenced on a NextSeq 550 (Illumina) system.

    Article Title: Ionic Regulation of Mechanosurveillance and Metastasis via the MRTFA/KCNMB1 Axis
    Article Snippet: The concentration of the final library pool was confirmed by qPCR and subjected to a test sequencing run on a MiniSeq instrument (Illumina) in order to check sequencing efficiencies and adjust accordingly proportions of individual libraries. .. The adjusted library pool was re-quantified by qPCR using Library Quantification Kit (Kapa Biosystems) and sequenced on the NovaSeq 6000 instrument (Illumina), flowcell, 2×150 bp reads. ..

    SYBR Green Assay:

    Article Title: A disease model resource reveals core principles of tissue-specific cancer evolution.
    Article Snippet: After each PCR step, solid-phase reversible immobilization clean-up (0.8×) was performed using an Agencourt AMPure XP kit (Beckman Coulter). .. The pooled library was quantified by SYBR Green qPCR (Sigma-Aldrich) and a Kapa Biosystems library quantification kit (Roche). .. The resulting library was sequenced on a NextSeq 550 (Illumina) system.

    Polymerase Chain Reaction:

    Article Title: Adenine base editors with reduced off-target effects
    Article Snippet: Stranded RNA-seq libraries were generated from these samples using the PrepX mRNA 48 kit (Takara) on the Apollo 324 followed by barcoding and amplification (12 cycles). .. Following PCR and bead cleanup with AmpureXP beads (Beckman Coulter), libraries were visualized on a 2200 TapeStation (Agilent) and quantified using a Library Quantification Kit (KAPA Biosystems) for multiplexing. libraries were sequenced on a NextSeq high-throughput flowcell (Illumina) as 150 bp paired-end reads. ..

    Multiplexing:

    Article Title: Adenine base editors with reduced off-target effects
    Article Snippet: Stranded RNA-seq libraries were generated from these samples using the PrepX mRNA 48 kit (Takara) on the Apollo 324 followed by barcoding and amplification (12 cycles). .. Following PCR and bead cleanup with AmpureXP beads (Beckman Coulter), libraries were visualized on a 2200 TapeStation (Agilent) and quantified using a Library Quantification Kit (KAPA Biosystems) for multiplexing. libraries were sequenced on a NextSeq high-throughput flowcell (Illumina) as 150 bp paired-end reads. ..



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